Technical Data
E3440-11A
Epstein Barr Virus, Nuclear Antigen (EBV)
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Technical Data |
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E3440-11A |
Epstein Barr Virus, Nuclear Antigen (EBV) |
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Description: Applications: Suitable for use in Western Blot, Immunofluorescence and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:10-1:50 (unboiled samples in 1mM DTT, 2% SDS). Immunofluorescence: 1:10-1:50. Note: Three step amplification procedure must be used. ELISA: 1:20-1:200 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Immunofluorescence: 1. Slides/Fixation: Cells should be suspended with PBS, pH 7.8, 200mM sodium chloride. Air dried onto slides at RT. Immediately fixed in-20ºC Methanol:Acetone (3:7). Air dry slides. Store @ 4ºC (<1 month) or at -20ºC (6-12 months) 2. Staining: Note: Three step amplification procedure must be used, i.e. Mab + goat anti-mouse IgG + Rabbit anti-goat labeled with FITC; or Mab + goat anti-mouse labeled with biotin + streptavidin labeled with FITC; or Mab + guinea pig complement + goat anti-guinea pig C3 labeled with FITC. Each incubation should be for 45 minutes at 37ºC. 3. Comments: A two step staining procedure is not sensitive enough to detect the EBNA. This is not a function of the monoclonal antibody, but rather a result of the small quantity of EBNA present in the cells. |
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