Technical Data
E3440-11A
Epstein Barr Virus, Nuclear Antigen (EBV)
Description:
Applications:
Suitable for use in Western Blot, Immunofluorescence and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:10-1:50 (unboiled samples in 1mM DTT, 2% SDS).
Immunofluorescence: 1:10-1:50. Note: Three step amplification procedure must be used.
ELISA: 1:20-1:200
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunofluorescence:
1. Slides/Fixation:
Cells should be suspended with PBS, pH 7.8, 200mM sodium chloride.
Air dried onto slides at RT.
Immediately fixed in-20C Methanol:Acetone (3:7).
Air dry slides.
Store @ 4C (<1 month) or at -20C (6-12 months)
2. Staining:
Note: Three step amplification procedure must be used, i.e. Mab + goat anti-mouse IgG + Rabbit anti-goat labeled with FITC; or Mab + goat anti-mouse labeled with biotin + streptavidin labeled with FITC; or Mab + guinea pig complement + goat anti-guinea pig C3 labeled with FITC.
Each incubation should be for 45 minutes at 37C.
3. Comments:
A two step staining procedure is not sensitive enough to detect the EBNA. This is not a function of the monoclonal antibody, but rather a result of the small quantity of EBNA present in the cells.
TypeIsotypeCloneGrade
MabIgG12Q1914Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Epstein Barr Virus, Nuclear Antigen.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Specific for the Epstein Barr Nuclear Antigen (EBNA-1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.