Technical Data
E3440-32D1
Epstein Barr Virus (EBV), Latent Membrane Protein (Mixed clones) (LMP)
Description:
EBV is a human Herpes virus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. LMP1 can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen and paraffin): 1:100-1:200.
Requires protein digestion pre-treatment of paraffin sections (e.g. trypsin). Optimal dilutions to be determined by the researcher.

Hybridoma:
3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1Mixed clonesSupernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Recombinant fusion protein of bacterial beta-galactosidase and EBV LMP-1
Purity:
Supernatant
Form
Supplied as a lyophilized powder, 15mM Sodium Azide. Reconstitute with 1ml ddH2O.
Specificity:
A cocktail of 4 antibodies recognizing different epitopes of Epstein Barr Virus LMP. This product stains EBV positive lymphoblastoid cell lines and EBV infected B cells in infectious mononucleosis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Murray, P.G., et al., (1992), "Immunohistochemical demonstration of the Epstein Barr virus-encoded latent membrane protein in paraffin sections of Hodgkin's disease", J. Pathol., 166: 1-5.