Technical Data
E3440-50A
EBV-Induced Gene 3 (EBI3, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein)
Description:
Epstein-Barr virus-induced gene protein 3 (EBI3), also known as Interleukin-27 beta chain (IL27B) is a widely expressed homolog to the interleukin IL-12p40 subunit protein. It forms a heterodimer with either IL-12p35 or an IL-12p35 homologue, IL27-p28 (also called IL-28), to create a new cytokine (IL-27). IL-27 plays an important function in regulating the activity of B and T lymphocytes. It drives rapid clonal expansion of naive CD4(+) T-cells and strongly synergizes with IL-12 to trigger IFN-gamma production of naive CD4(+) T-cells. The human Ebi3 encodes a secreted glycoprotein that is expressed in the spleen and tonsils, and at high levels in full-term placenta.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:32000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide with sequence C-IRYKRQGAARFHR, from the internal region of human EBI3 (NP_005746.2).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human EBI3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Collison LW, Workman CJ, Kuo TT, Boyd K, Wang Y, Vignali KM, Cross R, Sehy D, Blumberg RS, Vignali DA. The inhibitory cytokine IL-35 contributes to regulatory T-cell function. Nature. 2007 Nov 22;450(7169):566-9. PMID: 18033300