Technical Data
EBI3 (Interleukin-27 Subunit beta, IL-27 Subunit beta, IL-27B, Epstein-Barr Virus-induced Gene 3 Protein, EBV-induced Gene 3 Protein, IL27B)
EBI3 is a novel hematopoietin receptor family member related to the p40 subunit of interleukin-12 and to the ciliary neurotrophic factor receptor. It's expression is induced in B lymphocytes by Epstein-Barr virus (EBV) infection. Human EBI3 gene maps to chromosome 19p13.3, near genes encoding the erythropoietin receptor and the cytokine receptor-associated kinase, Tyk2. The protein is expressed in vivo by scattered cells in interfollicular zones of tonsil tissue, by cells associated with sinusoids in perifollicular areas of spleen tissue, and at very high levels by placental syncytiotrophoblasts. EBI3 expression in vitro is induced in EBV-negative cell lines by expression of the EBV latent infection membrane protein-1 and in peripheral blood mononuclear cells by pokeweed mitogen stimulation. The protein plays a crucial role in regulating cell-mediated immune responses.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-6ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa50-100 of human EBI3.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes human EBI3. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Devergne, O. et al. J. Virol. 70:1143-1153 (1996). 2. Odile, D. et al. Proc. Natl. Acad. Sci. USA 94:12041-12046 (1997).