Technical Data
E3451-26N
ErbB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, CD340, p185erbB2, HER2, MLN19, NEU, NGL)
Description:
HER2 has been implicated in numerous functions including normal and aberrant development in a variety of tissues. It is a ligand-less tyrosine kinase receptor of the ErbB family that is frequently overexpressed in breast cancer. It has been shown that HER2 undergoes proteolytic cleavage resulting in the release of the extracellular domain and the production of a phosphorylated membrane bound domain.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
SK-OV3 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human HER2 around the phosphorylation site of tyrosine 1221/1222 (N-L-YP-YP -W).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human HER2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Molina, MA. et al. J. Cancer. Res. 61:4744-4749 (2001). 2. Zou, W. et al. Oncogene. 20:3918-3920 (2001).