Technical Data
E3451-27
ErbB2, p185 (c-erbB2, HER2, neu Oncoprotein)
Description:
The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500 detects c-erbB-2 on 10ug of HEK293 lysates.
Immunohistochemistry: Reported to detect c-erbB-2 in frozen and formalin/paraffin sections of rat breast.
Immunoprecipitation: 4ug immunoprecipitated c-erbB-2 from 600ug of MCF-7 RIPA lysate and previously in A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysates.
Optimal dilution determined by the researcher.

Recommended Control:
A431 cells, HEK293, Jurkat, MCF7 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243- 1255 from the human c-erbB-2/HER-2/neu oncoprotein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes human c-erbB-2 (185 kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4. Species Crossreactivity: monkey rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Martin, L. A., et al. (2003). J Biol Chem. 278: 30458-30468. 2. Park, S. K., et al. (2001). J Cell Biol. 154: 1245-58. 3. Ezeh, P. I. and Farbman, A. I. (1998). J Neurobiol. 37: 199-210.
4. Press MF, et al. (1993). Cancer Res. 53:4960. 5. Paik, et al. (1990). J. Clin. Oncol. 8:103.