Technical Data
E3451-27A
ERBB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, p185erbB2, CD340, HER2, MLN19, NEU, NGL)
Description:
HER2/neu (also known as ErbB-2, ERBB2) is a protein (protein accession # P04626; 1255aa, ~185kD, chromosome 17q21.1) highly expressed in breast cancers. It is a cell membrane surface- bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. The oncogene neu is so-named because it was derived from a neuroglioblastoma cell line in rat. ErbB2 was named for its similarity to ErbB (avian erythroblastosis oncogene B). Herstatin, as the product of alternative HER-2 transcript, retains intron 8. The herstatin mRNA is expressed in normal human fetal kidney and liver, but is at reduced levels relative to p185HER-2 mRNA in carcinoma cells that contain an amplified HER-2 gene. Herstatin appears to be an inhibitor of p185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage-independent growth of transformed cells that overexpress HER-2.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml (ECL)
ELISA: 1:10,000-1:150,000 (10-100ng of control peptide/well).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b11C99Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Purified recombinant protein corresponding to aa23-652 at the extracellular domain of human ErbB2. (Accession #P04626).
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% Trehalose. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes human ErbB2. Does not react with human EGFR, Erbb3, or ErbB4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yamamoto T (1986) Nature 319, 230-234. 2. Semba K (1985) PNAS 82, 6497-6501. 3. Akiyama T (1986) Science 232, 1644-1646. 4. Bargmann CI (1986) Nature 319, 226-230. 5. Coussens L (1985) Science 230, 1132-1139. 6. Doherty JK (1999) PNAS 96, 10689-10874.