		Technical Data
E3451-27A	ERBB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, p185erbB2, CD340, HER2, MLN19, NEU, NGL)

Description:
HER2/neu (also known as ErbB-2, ERBB2) is a protein (protein accession # P04626; 1255aa, ~185kD, chromosome 17q21.1) highly expressed in breast cancers. It is a cell membrane surface- bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. The oncogene neu is so-named because it was derived from a neuroglioblastoma cell line in rat. ErbB2 was named for its similarity to ErbB (avian erythroblastosis oncogene B). Herstatin, as the product of alternative HER-2 transcript, retains intron 8. The herstatin mRNA is expressed in normal human fetal kidney and liver, but is at reduced levels relative to p185HER-2 mRNA in carcinoma cells that contain an amplified HER-2 gene. Herstatin appears to be an inhibitor of p185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage-independent growth of transformed cells that overexpress HER-2.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml (ECL)
ELISA: 1:10,000-1:150,000 (10-100ng of control peptide/well).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	11C99	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml (after reconstitution)

Immunogen:

Purified recombinant protein corresponding to aa23-652 at the extracellular domain of human ErbB2. (Accession #P04626).

Purity:

Purified by affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% Trehalose. Reconstitute with 100ul sterile PBS.

Specificity:

Recognizes human ErbB2. Does not react with human EGFR, Erbb3, or ErbB4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Yamamoto T (1986) Nature 319, 230-234. 2. Semba K (1985) PNAS 82, 6497-6501. 3. Akiyama T (1986) Science 232, 1644-1646. 4. Bargmann CI (1986) Nature 319, 226-230. 5. Coussens L (1985) Science 230, 1132-1139. 6. Doherty JK (1999) PNAS 96, 10689-10874.