Technical Data
E3451-49
ERC1, NT (ELKS/Rab6-interacting/CAST Family Member 1, ERC-1, Cast2, ELKS, FLJ31750, KIAA1081, MGC12974, Rab6-interacting Protein 2, RAB6IP2)
Description:
ERC1 (ELKS/Rab6­interacting/CAST family member 1), otherwise known as ELKS, is a widely expressed member of the RIM­binding protein family, which can be recruited on Golgi membranes by RAB6A, and may be involved in Rab­6 regulated endosomes to Golgi transport.
ERC1 is as an essential regulatory subunit of the IkappaB kinase (IKK) complex, a key enzyme in activation of nuclear factor kappaB (NF­kappaB). ERC1 is highly expressed in the brain, testis and thyroid, and is implicated in the formation of the cytomatrix at the active zone (CAZ), believed to define the site of calcium­dependent exocytosis of neurotransmitters.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:128,000
Western Blot: 0.1-0.3ug/ml detects a band of ~90kD in human pancreas cell lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to YGSARSVGKVEPS­C from the N­Terminal region of ERC1 (NP_829883.1; NP_829884.1).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognises an epitope within the N­terminal (NT) region of human ERC1. Species sequence homology: canine, bovine, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.