Technical Data
E3455-07A
Erythropoietin, alpha, Recombinant, Human (Powder) (EPO, EPO-a, EPO-alpha, Epoetin, EP, MGC138142)
5ug
50ug
Growth Factors, Cytokines Storage: -20CShipping: Dry Ice
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166aa and having a predicted molecular mass of 21kD and apparent glycosylated molecular mass of 36-40kD.

Specific Activity:
Measured using Normocythaemic mice and was found to be 150,000 IU/mg

Amino Acid Sequence:
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
36-40kD
Source: Chinese hamster ovary (CHO) cells
Purity: 98% by RP-HPLC and SDS-PAGE. Chromatographically purified.
Concentration: ~0.1mg/ml

Form: Supplied as a lyophilized powder from a sodium citrate, sodium chloride, citric acid buffer. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of >0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ford PA, Ford JM, (2004) Transfusion Dec 44(12 Suppl):15S-25S. 2. Wei X, Swanson SJ, (2004) J Immunol Methods Oct 293(1-2):115-26. 3. Godin M, (2004) Soins Sep (688 Suppl):5. 4. Cervantes F, Alvarez-Larran A, Sureda A, (2004) Br J Haematol Nov 127(4):399-403. 5. Mesjasz J, Wiecek A, Mazur W, (2004) Pol Merkuriusz Lek Apr 16(94):362-7. 6. Bergstrom S, Ahlgren J, (2004) Lakartidningen Oct 7 101(41):3160-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.