Technical Data
E3455-09
Erythropoietin, beta, Recombinant, Human (EPO)
20ug
Molecular Biology Storage: -20CShipping: RT
Recombinant Human Erythropoietin-beta is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21kD and apparent glycosylated molecular mass of 30.4kD.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
Erythropoietin is fully biologically active when compared to standard. The Specific Activity was measured by Normocyth-aemic mice and was found to be 1.2x10e5IU/mg.

Endotoxin:
0.1ng/ug (IEU/ug) of EPO-b.

Reconstitution:
Reconstitute the lyophilized EPO-b in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: CHO cells
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophlilzed powder in sodium phosphate dibasic, sodium phosphate monobasic buffer, 60% sodium chloride, 0.6% calcium chloride and 0.004% Tween 20.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1.Shinohara K, Mitani N, Sakuragi S, Kaneoka H, (2005) Am J Hematol Jan;78(1):15-20 2. Silverberg DS, Wexler D, Iaina A, Schwartz D, (2005) Kidney Blood Press Res 28(1):41-7. 3. Aapro M, San Miguel J, (2004 ) Oncology 67(Suppl 1):17-22. 4. Reed N, Morere JF, (2004) Oncology 67(Suppl.1):12-6. 5. Bogdanos J, Karamanolakis D, Repousis P, Tsintavis A, (2004) Anticancer Res May-Jun;24(3b):1957-61. 6. Tolman C, Duja S, Rashid R, Will E, (2004) Nephrol Dial Transplant Aug;19(8):2133-6.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.