Technical Data
E3500-04J
Escherichia coli (E. coli)
Description:
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae-bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

Applications:
Suitable for use in ELISA, Dot Blot and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceE. coliGoat
Concentration:
~1mg/ml
Immunogen:
Heat inactivated blend of 6 different strains of E. coli: TOP 10F, HB101, JM109, BL21, DH5 alpha and K12.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in Citrate/HEPES buffer, pH 7.2
Specificity:
Recognizes all E. coli species in immunogen, plus O157-H7. Recognizes more than 50 proteins by Western Blot.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Smith, G.P. and Gingrich, T.R., (2005) BioTechniques 39; 879-883.