Technical Data
E3500-26
Escherichia coli (E. coli)
Description:
Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine. E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Matched Pair:
Capture: E3500-25; Escherichia coli, O157:H7, Pathogenic (E. coli)
Detection: E3500-26; Escherichia coli (E. coli)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ul-20°CBlue IceRabbit
Concentration:
~5mg/ml
Immunogen:
Whole cell preparation of E. coli, all serotypes.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide
Specificity:
Recognizes all O and K antigenic serotypes of E. coli.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
General References: 1. "Escherichia coli O157:H7". CDC Division of Bacterial and Mycotic Diseases. Retrieved on 2007-01-25. 2. Vogt, R.L. & Dippold, L., Public Health Rep. 120(2): 174–178 (2005). 3. Bentley, R. & Meganathan, R., Microbiol. Rev. 46(3): 241–280 (1982). 4. Hudault, S., et al., Gut 49(1): 47–55 (2001). 5. Reid, G., et al., Trends Microbiol. 9(9): 424–428 (2001). 6. Feng, P., et al., (2002-09-01). "Enumeration of Escherichia coli and the Coliform Bacteria". Bacteriological Analytical Manual (8th ed.). FDA/Center for Food Safety & Applied Nutrition. Retrieved on 2007-01-25. 7. Thompson, Andrea (2007-06-04). "E. coli Thrives in Beach Sands". Live Science. Retrieved on 2007-12-03. 8. "Escherichia". Taxonomy Browser. NCBI. Retrieved on 2007-11-30. 9. Kubitschek, H.E., J. Bacteriol. 172(1): 94–101 (1990). 10. Madigan, M.T. & Martinko, J.M., Brock Biology of microorganisms (11th ed.). Pearson (2006). ISBN 0-13-196893-9. 11. Fotadar, U., et al., J. Basic Microbiol. 45(5): 403–404 (2005). 12. Ingledew, W.J. & Poole, R.K., Microbiol. Rev. 48(3): 222–271 (1984). 13. Darnton, N.C., et al., J. Bacteriol. 189(5): 1756-1764 (2007). 14. Brüssow, H., et al., Microbiol. Mol. Biol. Rev. 68(3): 560–602 (2004).