		Technical Data
E3500-28J	Escherichia coli, J5 LPS (E. coli)

Description:
The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).

Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2b	10C141	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~0.5mg/ml

Immunogen:

E. coli J5 cells

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes E. coli J5 LPS. Species crossreactivity: K. pneumoniae, S. sonnei and S. typhimurium.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.