Technical Data
E3500-28J
Escherichia coli, J5 LPS (E. coli)
Description:
The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).

Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b10C141Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
E. coli J5 cells
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes E. coli J5 LPS. Species crossreactivity: K. pneumoniae, S. sonnei and S. typhimurium.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.