Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine. E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.
Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested.
Immunofluorescence: Acetone fixation of the antigen is recommended.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| As Reported|
|E. coli -all serotypes|
|Purified by chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).|
|Recognizes all O and K antigenic serotypes of E. coli. Note: This antibody is unabsorbed and will cross-react with related Enterobacteriaceae.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. "Escherichia coli O157:H7". CDC Division of Bacterial and Mycotic Diseases. Retrieved on 2007-01-25.
2. Vogt RL, Dippold L (2005). "Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002". Public Health Rep 120 (2): 174–8. PMID 15842119.
3. Bentley R, Meganathan R (01 September 1982). "Biosynthesis of vitamin K (menaquinone) in bacteria". Microbiol. Rev. 46 (3): 241–80. PMID 6127606. PMC: 281544.
4. Hudault S, Guignot J, Servin AL (July 2001). "Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection". Gut 49 (1): 47–55. doi:10.1136/gut.49.1.47. PMID 11413110.
5. Reid G, Howard J, Gan BS (September 2001). "Can bacterial interference prevent infection?". Trends Microbiol. 9 (9): 424–8. doi:10.1016/S0966-842X(01)02132-1. PMID 11553454.
6. Feng P, Weagant S, Grant, M (2002-09-01). "Enumeration of Escherichia coli and the Coliform Bacteria". Bacteriological Analytical Manual (8th ed.). FDA/Center for Food Safety & Applied Nutrition. Retrieved on 2007-01-25.
7. Thompson, Andrea (2007-06-04). "E. coli Thrives in Beach Sands". Live Science. Retrieved on 2007-12-03.
8. "Escherichia". Taxonomy Browser. NCBI. Retrieved on 2007-11-30.
9. Kubitschek HE (01 January 1990). "Cell volume increase in Escherichia coli after shifts to richer media". J. Bacteriol. 172 (1): 94–101. PMID 2403552. PMC: 208405.
10. Madigan MT, Martinko JM (2006). Brock Biology of microorganisms (11th ed.). Pearson. ISBN 0-13-196893-9.
11. Fotadar U, Zaveloff P, Terracio L (2005). "Growth of Escherichia coli at elevated temperatures". J. Basic Microbiol. 45 (5): 403–4. doi:10.1002/jobm.200410542. PMID 16187264.
12. Ingledew WJ, Poole RK (1984). "The respiratory chains of Escherichia coli". Microbiol. Rev. 48 (3): 222–71. PMID 6387427.
13. Darnton NC, Turner L, Rojevsky S, Berg HC, On torque and tumbling in swimming Escherichia coli. J Bacteriol. 2007 Mar;18 Brüssow H, Canchaya C, Hardt WD (September 2004). "Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion". Microbiol. Mol. Biol. Rev. 68 (3): 560–602. doi:10.1128/MMBR.68.3.560-602.2004. PMID 15353570. PMC: 515249.