Technical Data
E3564-75
Estrogen Receptor (ER)
Description:
Applications: Affinity Purification (for conjugation, Ab can be ordered without BSA), Gel Supershift (Ab should be ordered at 1mg/ml), Immunoprecipitation. Not recommended for
Immunohistochemistry, Western Blotting.

Recommended Dilution:
Immunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate
Optimal dilutions to be determined by the researcher.
TypeIsotypeCloneGrade
MabIgG1,l0.N.282Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceBovineMouse
Concentration:
200ug/ml
Immunogen:
Purified, SDS-denatured calf uterus estrogen receptor.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide at 1mg/ml, cat# E3564-75X.
Specificity:
Recognizes Estrogen Receptor (ER) at 67kDa. The epitope maps between residues 120-170 (I group) of the B domain of human ER. Species Reactivity: Human, bovine, Rabbit, Mouse and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Abbondanza C et. al., (1993) Steroids 58:4-12.