Technical Data
E3564-85
Estrogen Receptor (ER)
Description:
ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Applications:
Suitable for use in Immunohistochemistry (paraffin tissues) and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: :50-1:100 for 2 hrs at RT
Immunohistology (Formalin/paraffin): 1:100-1:200 for 30 min at RT. Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Positive Control: MCF-7 cells. Breast Carcinoma.

Cellular Localization: Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1+IgG1,k3F173 + 5G102Supernatant
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant human estrogen receptor protein.
Purity:
Culture supernatant
Form
Supplied as a liquid with 0.09% sodium azide.
Specificity:
Recognizes Estrogen Receptor. Strongly stains the nucleus of epithelial cells in breast carcinomas. Species Crossreactivity: Human and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Al Saati, et al. (1993) Int J Cancer, 55:651-654. 2. Abbondanza C et. al., (1993) Steroids 58:4-12.