Technical Data
E3565-06D
Estrogen Receptor (ER)
Description:
ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-1.0ug/ml for 2hrs at RT
Immunohistochemistry (formalin/paraffin): 0.4-0.8ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher.

Positive Control:
MCF-7 cells, Breast carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F173Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Recombinant human estrogen receptor protein. Epitope: N-terminal domain.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3565-06DX
Specificity:
Recognizes human Estrogen Receptor. Strongly stains the nucleus of epithelial cells in breast carcinomas. Species Crossreactivity: monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Al Saati, et al. (1993) Int J Cancer, 55:651654.