Technical Data
E8445-50
ETFDH, NT (Electron Transfer Flavoprotein-ubiquinone Oxidoreductase, Mitochondrial, ETF-ubiquinone Oxidoreductase, ETF-QO, Electron-transferring-flavoprotein Dehydrogenase, ETF Dehydrogenase)
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Technical Data |
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E8445-50 |
ETFDH, NT (Electron Transfer Flavoprotein-ubiquinone Oxidoreductase, Mitochondrial, ETF-ubiquinone Oxidoreductase, ETF-QO, Electron-transferring-flavoprotein Dehydrogenase, ETF Dehydrogenase) |
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Description: Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67-kD precursor which is targeted to mitochondria and processed in a single step to a 64-kD mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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