Technical Data
E8445-50
ETFDH, NT (Electron Transfer Flavoprotein-ubiquinone Oxidoreductase, Mitochondrial, ETF-ubiquinone Oxidoreductase, ETF-QO, Electron-transferring-flavoprotein Dehydrogenase, ETF Dehydrogenase)
Description:
Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67-kD precursor which is targeted to mitochondria and processed in a single step to a 64-kD mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human ETFDH (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human ETFDH.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Olsen,R.K., et.al., Hum. Mutat. 22(1), 12-23 (2003).