Technical Data
E8445-50A
ETFDH (Electron Transfer Flavoprotein-ubiquinone Oxidoreductase, Mitochondrial, ETF-ubiquinone Oxidoreductase, ETF-QO, Electron-transferring-flavoprotein Dehydrogenase, ETF Dehydrogenase)
Description:
Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67kD precursor which is targeted to mitochondria and processed in a single step to a 64kD mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000
Western Blot: 1-3ug/ml, observed in human, mouse and rat heart lysates on ~70kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-EHDQPAHLTLRD, from ETFDH, at the internal region of the protein (NP_004444.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human ETFDH. Species Crossreactivity: Mouse and rat. Species sequence homology: Bovine, canine and rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Illig T, Gieger C, Zhai G, Römisch-Margl W, Wang-Sattler R, Prehn C, Altmaier E, Kastenmüller G, Kato BS, Mewes HW, Meitinger T, de Angelis MH, Kronenberg F, Soranzo N, Wichmann HE, Spector TD, Adamski J, Suhre K, A genome-wide perspective of genetic variation in human metabolism. Nature genetics 2010 Feb 42(2):137-41.