Technical Data
E8490
Ethenoadenosine
Description:
The reaction of chloroacetaldehyde (CAA) with DNA results in the formation of ethenoadenosine also known as 1,N6-ethenodeoxy-adenosine or etheno-A. CAA is formed through metabolization of for example vinyl chloride, a well established carcinogen. In RNA, after in vitro activation, etheno-A and etheno-C are the principle products of RNA damage. Etheno derivate formation may be highest in single-stranded DNA-regions.

Applications:
Can be used for Immunoprecipitation, Western Blot and Flow Cytometry. Useful for Immunohistology on frozen sections and immunoassays.

Recommended Dilution:
Immunohistology: 1:10
Flow Cytometry: 1:10
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG26A544Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.
Specificity:
Reacts with both the ribose and deoxyribose form of the adduct. Not crossreactive with non-modified DNA or the normal nucleotides.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Young, T et al, (1988) Carcinogenesis 9:589. 2. Krebs, C et al, (2003) Anal Biochem 314:108.