		Technical Data
E8490-01A	Ethenoadenosine

Description:
Ethenobases are adduct products of vinyl chloride, an occupational carcinogen. The sensitivity and specificity of this ethenoadenosine antibody have been determined by enzyme-linked immunosorbent assay. It reacts with both the ribose and deoxyribose form of the adduct. In addition, previous lots of this antibody have been shown to detect etheno-ADP-ribosylation of cell surface proteins by flow cytometry.

Cellular Localization: Nuclear

Applications:
Suitable for use in Immunofluorescence, ELISA and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2,l	8k129	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

Ethenoadenosine.

Purity:

Purified by Protein G affinity chromatography.

Form

As reported

Specificity:

This antibody is specific for ethenoadenosine and ethenod-adenosine. Species Crossreactivity: all

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Krebs, C. et al, (2003) Anal Biochem. 314(1):108-115. 2. Young, TL. et al, (1988) Carcinogenesis. 9(4):589-592. 3. Grahnert, A. et al, ( 2002) Biochem. J. 362:717-723. 4. Ohlrogge, W. et al, (2002) Mol. and Cell Biol. 22(21):7535-7542. 5. Liao, S. D., and Puro, D. G. (2006) 47:5032-5038.