Technical Data
E8490-01A
Ethenoadenosine
Description:
Ethenobases are adduct products of vinyl chloride, an occupational carcinogen. The sensitivity and specificity of this ethenoadenosine antibody have been determined by enzyme-linked immunosorbent assay. It reacts with both the ribose and deoxyribose form of the adduct. In addition, previous lots of this antibody have been shown to detect etheno-ADP-ribosylation of cell surface proteins by flow cytometry.

Cellular Localization: Nuclear

Applications:
Suitable for use in Immunofluorescence, ELISA and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2,l8k129Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Ethenoadenosine.
Purity:
Purified by Protein G affinity chromatography.
Form
As reported
Specificity:
This antibody is specific for ethenoadenosine and ethenod-adenosine. Species Crossreactivity: all
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krebs, C. et al, (2003) Anal Biochem. 314(1):108-115. 2. Young, TL. et al, (1988) Carcinogenesis. 9(4):589-592. 3. Grahnert, A. et al, ( 2002) Biochem. J. 362:717-723. 4. Ohlrogge, W. et al, (2002) Mol. and Cell Biol. 22(21):7535-7542. 5. Liao, S. D., and Puro, D. G. (2006) 47:5032-5038.