Technical Data
Media
Ethyl Violet Azide Broth (Powder)
E8608
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest Peptone13.5
Dextrose5.0
Yeast Extract6.5
Sodium Chloride5.0
Dipotassium Phosphate2.7
Monopotassium Phosphate2.7
Sodium Azide0.4
Ethyl Violet0.83mg
Total:35.8g/liter
A selective confirmatory medium used to detect Enterococci in water.

Description:
This medium is highly selective for enterococci due to the presence of sodium azide and ethyl violet, which inhibit Gram negative bacilli and Gram positive sporeformers. The medium contains a phosphate buffer system for pH control. Peptone, yeast extract and dextrose provide the nutrients that support the growth of enterococci.

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions: 1. Dissolve 35.8grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi) Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Light to medium gold, clear, complete

pH (25C): 7.00.2

Microbiological Analysis: Enterococcus faecalis ATCC 29212

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.