		Technical Data
E8949-01A	Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)

Description:
EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2alpha. Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis. Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses. EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52kD).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	7H20	Ascites

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

Not Determined

Immunogen:

Purified recombinant human eIF2a

Purity:

Ascites

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes endogenous levels of total human eIF2a protein. Species Crossreactivity: mouse, rat and monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31:25-29. 2. De Haro, C. et al. (1996) FASEB J. 10:1378-1387. 3. Kaufman, R.J. (1999) Genes Dev. 13:1211-1233. 4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18:6121-6128. 5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274:4801-4806. 6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20:1278-1290.