		Technical Data
E8949-45M	4E-T (Eukaryotic Translation Initiation Factor 4E Transporter, eIF4E Transporter, Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1, EIF4ENIF1)

Description:
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic;its own nuclear import is regulated by a nuclear localization signal and nuclear export signals.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.5ug/ml
Immunohistochemistry (paraffin): 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
293 lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to CAKVISVDELEYRQ, from the C-terminus of the protein sequence according to NP_062817.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Specificity:

Recognizes human 4E-T. Species sequence homology: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N. A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E. EMBO J. 2000 Jun 15;19(12):3142-56. PMID: 10856257