Technical Data
E9009-31A
Exportin-1 (Exp1, Chromosome Region Maintenance 1 Protein Homolog, XPO1, CRM1)
Description:
Nuclear transport receptors of karyopherin:importin-beta superfamily mediate various transport pathways between nucleus and cytoplasm. Exportin-1, a human homolog of yeast CRM1, is a novel karyopherin export receptor that mediates leucine-rich NES-dependent protein export. It interacts with RanGTP through its Ran-binding domain and also with nucleoporins including Nup214, Nup50, Nup42 and Nup159 during the process of exporting NES-containing proteins. It mediates the export of many cellular and viral proteins and ribonucleoproteins including HIV Rev Protein, ERK3, PKI, IRF-5, Cyclin-B, MAPKK, MAPKAP kinase 2, Snurportin, Cap-binding protein, and certain U snRNAs. Reports suggest exportin-1 also has a role in the regulation of NFAT and AP-1.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa390-408 (ASPLLSGSQHFDVPPRRQL) of human Exportin-1.
Purity:
Purified by Protein G affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human Exportin-1. Species Crossreactivity: chimpanzee, bovine, canine. Species sequence homology: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fornerod et.al Cell 90 (6) 1051-1060 (September 19, 1997) 2. Petosa et.al Mol Cell 16 (5) 761-775 (December 3, 2004) 3. Julien et.al JBC 278 (43) 42615-42624 (October 24, 2003).