Technical Data
E9100-01B
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)
Description:
ERM (Ezrin-Radixin-Moesin) proteins function as plasma membrane-actin cytoskeleton linkers and participate in the formation of specialized domains of the plasma membrane. One function of ezrin is in determining survival of epithelia cells by activating the P1-3-kinase/Akt pathway.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-1ug/ml detected Ezrin in RIPA lysates from A431 cells and a mourine brain lysate.
Immunohistochemistry: 10ug/ul detects Ezrin in paraf n embedded human skin tissue sections. Optimal dilutions to be determined by the researcher.

Positive Control Included:
A0001-50: A431 Cell Lysate, Non-Stimulated. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml.
Immunogen:
Synthetic peptide corresponding to amino acids 479-498 of human Ezrin (C-EPVSYHVQESLQDEGAEPTG). KLH-conjugated.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes human Ezrin, Mr 81kD. Species Crossreactivity: Mouse. Predicted to cross-react with rat based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Tsukita, S. and S. Yonemura, (1999) JBC, 274:34507.