Technical Data
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)
Erzin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton (1-2). Ezrin activity is regulated by intramolecular interactions between N- and C-terminal ERM association domains (3). Phosphorylation at threonine 567 is a critical regulator of ezrin function allowing the active protein to link target molecules to the actin cytoskeleton (4). Erzin tyrosine phosphorylation can also be induced by EGF, PDGF and HGF stimulation. Erzin interacts with PI3-K protein kinase A and Rho (5).

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:80
Western Blot: 1:5,000-50,000
Immunohistochemistry: 100-250
Immunocytochemistry: 50-100
Immunoprecipitation: 1:50

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul -20°CBlue IceHumanRabbit
A synthetic peptide corresponding to residues near the C-terminus of human Ezrin
Supplied as a liquid.
Recognizes human Ezrin
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Bretscher, A., et al., (2002) Nat. Rev. Mol. Cell. Biol. 3:586–599. 2.Tsukita, S., and Yonemura, S. (1999) J. Biol. Chem. 274:34507–34510. 3.Pearson, M. A., Reczek, D., Bretscher, A., and Karplus, P. A. (2000) Cell 101:259-270. 4.Gautreau, A., Louvard, D., and Arpin, M. (2000) J. Cell Biol. 150:193-203. 5.Bretscher, A., Chambers, D., Nguyen, R., and Reczek, D. (2000) Annu. Rev. Cell Dev. Biol. 16:113–143.