Technical Data
E9100-03C
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing Extension Spike Protein)
Description:
Erzin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton (1-2). Ezrin activity is regulated by intramolecular interactions between N- and C-terminal ERM association domains (3). Erzin tyrosine phosphorylation can also be induced by EGF, PDGF and HGF stimulation. Erzin interacts with PI3-K protein kinase A and Rho (4). In response to epidermal growth factor (EGF) the microvillar core protein ezrin is phosphorylated transiently to a high level on tyrosine residues in human epidermoid carcinoma A431 cells. Tyr145, lies in the N-terminal region of homology that is common to the band 4.1-talin-ezrin protein family. This tyrosine residue and its vicinal amino acids are conserved throughout the family members, including radixin, moesin, and the two phosphotyrosine phosphatases, PTP H1 and PTP MEG, but not in band 4.1 or talin (5).

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:20
Western Blot: 1:1000-5000
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E296Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues surrounding tyrosine 145 of human Ezrin.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes both phosphorylated and nonphosphorylated human Ezrin. Species Crossreactivity: Mouse and rat (both WB only).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bretscher, A., et al., Nat. Rev. Mol. Cell. Biol. 3: 586–599 (2002). 2. Tsukita, S., et al., J. Biol. Chem. 274: 34,507–34,510 (1999). 3. Pearson, et al., Cell 101: 259-270 (2002). 4. Gautreau, A., et al., J. Cell Biol. 150: 193-203 (2000). 5. Krieg, J., et al., J. Biol. Chem. 267(27): 19,258-19,265 (1992).