		Technical Data
E9100-20	Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)

Description:
Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells.
It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Applications:
Suitable for use in Immunohistochemistry (Formalin-fixed paraffin-embedded sections and Frozen sections) and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50-1:100 for 30mins at RT (ABC method). For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with Immunohistochemistry staining.
Optimal dilutions to be determined by the researcher.

Positive Control:
Lung

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	4i251	Purified

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Recombinant fragment, corresponding to amino acids 362-585 of Human Ezrin.

Purity:

IgG fraction.

Form

Supplied as a liquid in PBS with sodium azide.

Specificity:

Detects a protein of 81kD corresponding to human Ezrin. Species Crossreactivity: Rat, Mouse, Bovine, Hamster, Monkey and Kangaroo.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bohling T et al., (1996) Am J Pathol 148:367-73. PubMed: 8579099. 2. Helander TS et al. (1996) Nature 382:265-8. PubMed: 8717043. 3. Turunen O, et al., (1994) J Cell Biol 126:1445-53. PubMed: 8089177.