		Technical Data
E9100-25A	Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)

Description:
The ezrin, radixin and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation (1). ERM proteins undergo intra-or intermolecular interaction between their amino-and carboxy-terminaldomains, existing as inactive, cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin), which disrupts their amino-and carboxy-terminal association, may play a key role in modulating the conformation and function of ERM proteins (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogeneinduced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide (KLH-coupled) corresponding to human ezrin.

Purity:

Purified by Protein A and immunoaffinity chromatography.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5,, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

Specificity:

Detects endogenous levels of total ezrin protein. Does not crossreact with ezrin homologues such as radixin and moesin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274:34507–34510. 2. Mangeat, P. et al. (1999) Trends Cell Biol. 9:187–192. 3. Matsui, T. et al. (1998) J. Cell Biol. 140:647–657. 4. Gautreau, A. et al. (2000) J. Cell Biol. 150:193–203. 5. Tran Quang, C. et al. (2000) EMBO J. 19:4565–4576. 6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:7300–7305.