Technical Data
E9100-25E
Ezrin (Cytovillin, Villin-2, p81, EZR, VIL2)
Description:
Ezrin is a member of the ERM family (ezrin, radixin, moesin) of proteins that link the cell membrane to the actin cytoskeleton and that are involved in cytoskeletal organization, cell motility and morphogenesis. The protein (586aa) consists of three domains: a membrane-binding aminoterminal domain, an extended alpha-helical domain, and a carboxyterminal domain that has an actin-binding site. Aberrantly high ezrin protein expression has been detected in the stromal cells of hemangioblastoma. Moreover, a high ezrin expression level is associated with immortalization and the increased proliferation rate of cells in vitro. It appears to play a role in the progression of tumors, such as the metastasis of osteosarcoma. Human Ezrin gene is localized to the end of the long arm of chromosome 6 (6q22-q27).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Placenta lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QDYEEKTKKAER, from the internal region of the protein sequence according to NP_003370.2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human Ezrin. Species sequence homology: canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Stanasila L, Abuin L, Diviani D, Cotecchia S. Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling. J Biol Chem. 2006 Feb 17;281(7):4354-63. Epub 2005 Dec 13. PMID: 16352594