		Technical Data
E9100-28D	Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)

Description:
Human moesin (membrane-organizing extension spike protein), a member of the ezrin, radixin, moesin (ERM) protein family, which acts as a linker protein between the plasma membrane
and the actin cytoskeleton, and as a signal transducer in responses involving cytoskeletal remodelling. Moesin is localized to cytoplasmic projections (filopodia) which extend from the leading edge of migrating cells, which play a key role in cell movement, signaling and cell-cell recognition.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16000
Western Blot: 1.0-3.0ug/ml, detects a band of ~70kD in human spleen cell lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Peptide sequence C-NARDESKKTAND from the internal region of moesin (NP_002435.1).

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in TBS, pH 7.2, 0.02% sodium azide, 0.5% BSA.

Specificity:

Recognizes human moesin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tsukita, S. et al. (1997) Cell Biol. 9:70-75.