Technical Data
FACT (cdc68, Facilitates Chromatin Transcription)
FACT complex is a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:2000 dilution detects FACT/cdc68 in HeLa nuclear extracts. HeLa nuclear extract lysate was resolved by electrophoresis, transferred to PVDF and probed with F0004. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP.
Immunoprecipitation: Reported to immunoprecipitate FACT from HeLa nuclear extracts.
Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
H1838: HeLa Nuclear Extract. Add an equal volume of 2X Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 min to reduce the preparation. Load 2-5ug of reduced lysate per lane for minigels.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceMouseRabbit
Not Determined
GST fusion protein corresponding to residues 802982 of mouse FACT/cdc68.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 0.2M Tris, pH 8.0, 0.15M sodium chloride, 0.1mM EDTA, 5mg/ml BSA, 0.05% sodium azide, before the addition of glycerol to 30%.
Recognizes mouse FACT/cdc68, Mr 140kD. A non-speci c band is detected at ~120kD in longer exposures or when more that 5ug of nuclear extract is loaded per lane. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Orphanides, G., et al., Nature 40: 284-288 (1999). 2. Yarnell, A.T., et al., J. Biol. Chem. 276: 25,736-25,741 (2001).