Technical Data
F0010-01B
Factor H (Complement Factor H, Beta 1H)
Description:
Human complement factor H exists in 2 forms. The most common form, of 150kD, and the less common form of 43kD. Factor H is secreted by the liver into the blood serum. It is important in regulating the complement pathway, preventing unnecessary inflammation which can damage the host tissue. Complement factor H functions as a cofactor in the inactivation of C3b by factor I. It makes C3b susceptible to cleavage by factor I, resulting in iC3b. Factor H also inhibits the formation of the C3bBb complex (C3 convertase) and increases the rate of dissociation of both C3 convertase and the (C3b)NBB complex (C5 convertase). This prevents these components of the classical and of alternative complement pathways from forming a positive feedback loop. Mutations in factor H are associated with hemolytic-uremic syndrome, age-related macular degeneration, membranoproliferative glomerulonephritis (MPGN) type II and chronic hypocomplementemic nephropathy.

Applications:
Suitable for use in ELISA, Immunohistochemistry, Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Positive Control:
Kidney from post streptococcal glomerulonephritis patients.

Recommended Secondary Antibodies:
I1904-75E: IgG1 (HRP) Pab RbxMo
I1904-79M: IgG1 (HRP) Pab GtxMo
I1904-75D: IgG1 (FITC) Pab RbxMo
I1904-76L: IgG1 (FITC) Pab GtxMo

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG17J1Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human complement factor H from serum.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in BBS, 0.1% sodium azide.
Specificity:
Recognizes human Complement Factor H at ~150kD or ~43kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Alexander, J., et al., Mol. Immunol. 44: 123-132 (2007). 2. Harrison, R., et al., Mol. Immunol. 17: 9-20 (1980).