Technical Data
F0010-01B
Factor H (Complement Factor H, Beta 1H)
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Technical Data |
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F0010-01B |
Factor H (Complement Factor H, Beta 1H) |
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Description: Human complement factor H exists in 2 forms. The most common form, of 150kD, and the less common form of 43kD. Factor H is secreted by the liver into the blood serum. It is important in regulating the complement pathway, preventing unnecessary inflammation which can damage the host tissue. Complement factor H functions as a cofactor in the inactivation of C3b by factor I. It makes C3b susceptible to cleavage by factor I, resulting in iC3b. Factor H also inhibits the formation of the C3bBb complex (C3 convertase) and increases the rate of dissociation of both C3 convertase and the (C3b)NBB complex (C5 convertase). This prevents these components of the classical and of alternative complement pathways from forming a positive feedback loop. Mutations in factor H are associated with hemolytic-uremic syndrome, age-related macular degeneration, membranoproliferative glomerulonephritis (MPGN) type II and chronic hypocomplementemic nephropathy. Applications: Suitable for use in ELISA, Immunohistochemistry, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Kidney from post streptococcal glomerulonephritis patients. Recommended Secondary Antibodies: I1904-75E: IgG1 (HRP) Pab RbxMo I1904-79M: IgG1 (HRP) Pab GtxMo I1904-75D: IgG1 (FITC) Pab RbxMo I1904-76L: IgG1 (FITC) Pab GtxMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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