Technical Data
F0010-01H
CFH (Complement Factor H, H Factor 1, HF, HF1, HF2)
Description:
Factor H (CFH) is a protein with twenty short consensus repeat (SCR) domains. Factor H is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in Factor H have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western BloProduct Type: Mab
Isotype: IgG1
Clone No: OX-24
Host: mouse
Source: human
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
Purity: Purified by Protein G affinity chromatography.
Immunogen: Human complement factor H
Specificity: Recognizes human serum complement factor H and a 43-49kD truncated form of factor H.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.t, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (FFPE): 20ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with F0010-01H, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1OX-24Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human complement factor H
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human serum complement factor H and a 43-49kD truncated form of factor H.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Chu H, et. al. 2014. Noninflammatory necrotizing vasculopathy in lupus nephritis: a single-center experience. Lupus. 23(1):20-30.