Technical Data
F0017
Factor IX
Description:
Human Factor IX is a glycoprotein which is synthesized in the liver (1–3). The domain structure of factor IX is similar to that of the other vitamin K dependent coagulation factors. The NH2-terminal region contains 12 g-carboxyglutamic acid (gla) residues which facilitate the calcium dependent binding of factor IX to negatively charged phospholipid surfaces. Two domains which are homologous to epidermal growth factor (EGF) span the region between the NH2-terminal gla domain and the activation peptide (Ala-146 to Arg-180).
Factor IX is activated by either factor XIa or the factor VIIa/tissue factor/phospholipid complex. Cleavage at site A yields the intermediate IXa which is subsequently converted to the fully active form IXab by cleavage at site B. The NH2-terminal light chain (GLA and EGF domains) remains covalently attached to the COOH-terminal heavy chain by a disulfide bond. The serine protease catalytic triad (Ser-365, His 221, Asp-269) is located in the heavy chain. Factor IXab is the catalytic component of the “intrinsic factor Xase complex” (factor VIIIa/IXa/Ca2+/phospholipid) which proteolytically activates factor X to factor Xa.

Applications:
Suitable for use in RIA, Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA:
Coating: 5-10ug/ml
Secondary: 100-1000ug/ml
Immunohistochemistry: 100ng/ml
Optimal dilutions to be determined by the researcher.

Extinction Coefficient (1%, 1cm, 280nm): 14.0

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18.H.1Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~10mg/ml
Immunogen:
Human Factor IX.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 50% glycerol.
Specificity:
Recognizes human Factor IX, human Factor IXa and heavy chain of human Factor IX and IXa.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.