Technical Data
F0019-50FX
Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor) (BSA & Azide Free)
Description:
Factor XIII is a beta-globulin found in plasma. It is composed of two subunits. Factor XIIIa is the catalytic subunit. It is a dimer of MW 160kD. Factor XIII is present in plasma as an a2b2 heterodimer (MW 320kD). In platelets, only the a2 unit exists. Factor XIIIa is a dermal dendrocyte marker. It shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system. Factor XIII has also been used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1:100 for 20 minutes at RT using the LP system, 30 minutes at RT using UltraVision. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Recombinant human Factor XIII a-subunit, capillary hemangioma, dermatofibroma and placenta.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3F177Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to a-subunit of coagulation Factor XIII. Cellular Localization: Cytoplasmic and nuclear. MW of Antigen: Unreduced dimer: 160kD, Reduced monomer: 80kD
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See F0019-50F.
Specificity:
Recognizes human reduced (80kD) and unreduced (160kD) forms of Factor XIII. Does not recognize human Factor XIII B-chain or human Factor XII.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.