Technical Data
F0019-50GX
Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor) (BSA, Azide & Glycerol Free)
Description:
Factor XIII is a beta-globulin found in plasma and is composed of two subunits. Factor XIIIa is the catalytic subunit and is a dimer of MW 160kD. Factor XIII is present in plasma as an a2b2 heterodimer (MW 320kD). Whereas in platelets, only the a2 unit exists. Factor XIIIa is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system. Factor XIII has also been used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 5-10ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 10-20ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human recombinant Factor XIIIa. Capillary hemangioma, dermatofibroma and placenta.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to A subunit of coagulation Factor XIII. MW of Antigen:160kD (Unreduced dimer), 80kD (Reduced monomer). Cellular Localization: Cytoplasmic and nuclear
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservatives and no stabilizing proteins added. Also available with BSA and azide. See F0019-50G.
Specificity:
Recognizes human Factor XIIIa, both reduced and non-reduced forms. Does not crossreact with human Factor XIII B-chain or human Factor XII.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.