Technical Data
F0019-55K
FAK phosphorylated (Tyr407) (Focal Adhesion Associated Protein Tyrosine Kinase 1)
Description:
Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that was discovered as a substrate for Src and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Phosphorylation of tyrosine 407 is activated by TGFb and Epithelial Mesenchyme Transition (EMT). Unlike other sites on FAK, tyrosine 407 is EGFR independent.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: A 1:1000 detected FAK phosphorylated on residue Tyr407 in lysate from FAK transfected CEF cells grown on fibronectin.
Optimal dilution to be determined by researcher.

Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to amino acid region encompassing the human, mouse, rat, and chicken phospho-FAK (Tyr407). Species Sequence Homology: mouse, rat and chicken.
Purity:
Purified by affinity chromatography.
Form
Suplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/mL BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes human phospho-FAK (Tyr407), Mr 125kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Le Boeuf F, Houle F, Huot J, Regulation of vascular endothelial growth factor receptor 2-mediated phosphorylation of focal adhesion kinase by heat shock protein 90 and Src kinase activities. J Biol Chem (2004) 279: 39175-39185. 2. Matkowskyj KA, et al., Expression of GRP and its receptor in well-differentiated colon cancer cells correlates with the presence of focal adhesion kinase phosphorylated at tyrosines 397 and 407. J Histochem Cytochem (2003) 51: 1041-1048.