Technical Data
F0019-56
FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
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Technical Data |
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F0019-56 |
FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) |
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Description: Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Immunocytochemistry: 10ug/ml shows positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde. Western Blot: 0.5-2ug/ml detects FAK in RIPA lysates from murine 3T3 and previously in human A431 RIPA cell lysate. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with F0019-56 (1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates FAK from 500ug of murine 3T3 RIPA lysate. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb I1904-40D: IgG, H&L (FITC) Pab Gt xRb I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb I1904-46N: IgG, H&L (FITC) Pab Gt xRb Positive Antigen Control: F4209 3T3 cell lysate; use 20mg per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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