Technical Data
F0019-56
FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.

Recommended Dilution:
Immunocytochemistry: 10ug/ml shows positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde.
Western Blot: 0.5-2ug/ml detects FAK in RIPA lysates from murine 3T3 and previously in human A431 RIPA cell lysate. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with F0019-56 (1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates FAK from 500ug of murine 3T3 RIPA lysate.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
I1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
I1904-46N: IgG, H&L (FITC) Pab Gt xRb

Positive Antigen Control: F4209
3T3 cell lysate; use 20mg per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
pGEX-derived fusion protein containing aa748-1053 of human FAK.
Purity:
Purified by Protein A chromatography
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes and is specific for human p125 FAK. Does not crossreact with Pyk2. Species Crossreactivity: mouse, rat and hamster. Does not crossreact wirh avian.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kanner, S., et al., Proc. Natl. Acad. Sci. USA 87: 3328, 1990.