Technical Data
F0019-56V
FAK, phosphorylated (Tyr397) (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
Description:
Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that was discovered as a substrate for Src and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase and activates cell migration and invasion.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry applications. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Primary chick embryo fibroblasts expressing human FAK and plated on fibronectin, and NIH3T3 +/- PDGF.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic phosphopeptide from the region of human FAK that contains tyrosine 397. Species Sequence Homology: chicken, rat.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes human FAK, phosphorylated (Tyr397). Species Crossreactivity: mouse, frog, and fly FAK.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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