Technical Data
F0019-58R
FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
Description:
FAK is a widely expressed non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. Regulation of FAK includes phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phophorylation occurs upon integrin engagement with fibronectin. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells begin to enter the M-phase of the cell cycle. In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK binds multiple signaling proteins including p130Cas, Graf, Grb2, Src family SH2 domains, and the p85 subunit of PI3-kinase. The epitope is localized in the C-terminus (amino acid residues 853-1052).

Applications:
Suitable for use in Immunofluorescence, Western Blotting, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:10-1:50
Western Blotting: 5.0 _g/mL with an incubation for 2 hours at RT
Immunoprecipitation: 10.0 _g/mg of protein lysate

Optimal dilutions to be determined by the researcher.

Recommended Positive Control: HUVEC, NIH3T3 cells, endothelial cells, or kidney tissue.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to amino acid residues 853-1052 from the C-terminal region of human FAK.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide
Specificity:
Recognizes a protein of Mr125kD, identified as focal adhesion kinase (FAK). Species Reactivity: Human, mouse, rat, canine and frog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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Cance, W.G. and E.T. Liu (1995) Protein kinases in human breast cancer. Breast Cancer Res. Treat. 35(1):105-114.
Hanks, S.K. and T.R. Polte (1997) Signaling through focal adhesion kinase. BioEssays 19(2):137-145.
Ilic, D., C.H. Damsky, and T. Yamamoto (1997) Focal adhesion kinase: at the crossroads of signal transduction. J. Cell Sci. 110:401-407.
Martin, G.M. (1996) Fak and focal adhesions. Jpn. J. Cancer Res. 87(3):1.
Morimoto, C. and K. Tachibana, K. (1996) Beta 1 integrin-mediated signaling in human T- cells. Hum. Cell 9(3):163-168.
Neet, K. and T. Hunter (1996) Vertebrate non-receptor protein-tyrosine kinase families. Genes Cells 1(2):147-169.
Otey, C.A. (1996) pp125FAK in focal adhesion. Internat. Rev. Cytol. 167:161-183.
Owens, L.V., L. Xu, R.J. Craven, G.A. Dent, T.M. Weiner, L. Kornberg, E.T. Liu, and W.G. Cance (1995) Overexpression of the focal adhesion kinase (p125FAK) in invasive human tumors. Cancer Res. 55(13):2752-2755.
Schlaepfer, D.D. and T. Hunter (1996) Signal transduction from the extracellular matrixa role for the focal adhesion protein-tyrosine kinase FAK. Cell Struct. Funct. 21(5):445-450.