Technical Data
F0019-58W1
Fanconi anemia D2 (FA4, FACD, FAD, FAD2, FANCD2, Fanconi anemia Complementation Group D, Fanconi Pancytopenia Type 4)
Description:
Fanconi anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANCD2, targeting nuclear foci containing the BRCA1 protein.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG8k150Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human FANCD2 fusion protein.
Purity:
Purified by Protein G affinity chromatography.
Form
As reported
Specificity:
This antibody is specific for FANCD2. Species Crossreactivity: Reacts with human FANCD2. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Strahdee, C.A., et al. Evidence for at least four fanconi anaemia genes including FACC on chromosome 9. Nature Genet. 1:196-198, 1992.
2. Timmers, C., et al. Positional cloning of a novel fanconi anemia gene, FANCD2. Molec. Cell. 7:241-248, 2001. (Western Blot, human)
3. Garcia-Higuera, I. et al. Interaction of the fanconi anemia proteins and BRCA1 in a common pathway. Molec. Cell. 7:249-262, 2001. (Western Blot, Immunoprecipitation, human)
4. Nakanishi, K. et al. Interaction of FANCD2 and NBS1 i nthe DNA damage response. Nature Cell Bio. 4:913-920, 2002.