Technical Data
F0019-58W4B
Fanconi anemia F (FANCF, FAF, MGC126856, Protein FACF)
Description:
Research indicates that FANCF is involved in DNA repair, either with post-replication repair or a cell cycle checkpoint function. FANCF has also been implicated in inter-strand DNA cross-link repair and in the maintenance of normal chromosome stability.

Defects in FANCF are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopaenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level, it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.

Applications:
Suitable for use in ELISA and Immunohistochemistry (paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 2.5ug/ml
ELISA: 1:15,000-1:60,000
Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Positive Control: Human kidney

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ugBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide corresponding to an internal aa sequence of human FANCF.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes human FANCF. Species Crossreactivity: chimpanzee.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.