Technical Data
F0019-58X7
Farnesyltransferase (FPTA, CAAX Box, GGTase-I-alpha)
Description:
Prenylation is a common intracellular posttranslational protein modification catalyzed by prenyltransferases. The best characterized among these prenyltransferases is the CAAX farnesyl transferase (2.5.1.21), which is alpha-beta heterodimeric enzyme. It catalyzes the attachment of a farnesyl group from farnesyl phosphate to cysteine residues at the fourth position from the C terminus of proteins that end in the CAAX box. FNTA or the alpha subunit of farnesyl transferase is shared by the geranyl-geranyl protein transferases. Apart from transfer of farnesyl and geranyl groups to proteins, FNTA has been proposed to regulate intracellular signaling pathways involving p21ras. Reports have shown that FNTA interacts with TGF-beta and activin-type I receptors.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse colon cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa150-250 of human farnesyltransferase.
Purity:
Purified by Protein G affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human Farnesyltransferase. Species Crossreactivity: chichen, chimpanzee, canine, rat. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang et.al Science 271; 1120-1122 (1996) 2. Lane et.al J Lipid Res 47; 681-699 (2006).