Technical Data
F0019-64C
Fas Ligand, Recombinant, Human (FASL) (BSA Free)
2ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
FasL is a type II membrane protein belonging to the TNF family of cytokines. FasL induces apoptosis when it binds to cells expressing the Fas antigen. FasL was first discovered on the cell surface of T cells and NK cells. Expressed on the cell surface of activated T cells, FasL kills T cells and activated B cells leading to down-regulation of the immune response. The full length of FasL contains 281 amino acid residues, consisting of the transmembrane domain, extracellular domain and the cytoplasmic region. A metalloproteinase cleaves the membrane-bound FasL to produce soluble FasL (sFasL), which exists as a homotrimer. The sFasL shows lower cytotoxic activity than membrane bound FasL. Recombinant Fas Ligand is a soluble 19.9kD protein containing 175 amino acid residues, comprising of only the extracellular domain of FasL.

Biological Activity:
Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is <10ng/ml, corresponding to a specific activity of >1x10e5 units/mg.

Recombinant FASL Sequence:
LQKELAELRE STSQMHTASS LEKQIGHPSP PPEKKELRKV AHLTGKSNSR SMPLEWEDTY GIVLLSGVKY KKGGLVINET GLYFVYSKVY FRGQSCNNLP LSHKVYMRNS KYPQDLVMME GKMMSYCTTG QMWARSSYLG AVFNLTSADH LYVNVSELSL VNFEESQTFF GLYKL

Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human FASL was expressed in CHO cells.
Purity: 98% by SDS-PAGE
Form: Supplied as a lyophillized powder from PBS, pH 7.2.
Reconstitution: Spin down product and reconstitute in water to a concentration of 0.1-1.0mg/ml.
Endotoxin: 0.1ng/ug (1EU/ug).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Mita E et al (1994) BBRC 204, 468-474; 2. Hakuno N et al (1996) Endocrinology 137, 1938-48; 3. Hahne M (1995) Intl. Immunol. 7, 1381-1386; 4. Suda T and Nagata S (1994) J Exp. Med. 179, 873-879; 5. Takahashi T et al (1994) Cell 76, 969-976

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.