Technical Data
F0019-75K
Fatty Acid Binding Protein, Heart (FABP)
Description:
FABP is a plasma marker of acute myocardial infarction (AMI). The plasma kinetics of FABP (15kD) closely resemble those of myoglobin in that elevated plasma concentrations are found within 2 hours after AMI and return to normal generally within 18 to 24 hours. The concentration of FABP in the skeletal muscle is 20 times lower than in cardiac tissue (for myoglobin the same content for cardiac and skeletal tissue), making FABP cardiac-specific in comparison to myoglobin. This makes FABP a useful biochemical marker for the early assessment or exclusion of AMI. FABP also appears a useful plasma marker for the estimation of myocardial infarct size.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Heart cells, recombinant human H-FABP

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16A548Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified human H-FABP.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA and 0.02% sodium azide.
Specificity:
Recognizes human heart FABP (native and recombinant).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Roos, W., et al., J. Immunol. Meth. 183: 149 (1995). 2. Guillame, E., et al., Proteomics 3: 1495 (2003). 3. Pelsers, M., et al., Clin. Chem. 50: 1568 (2004).