Technical Data
F0019-75K
Fatty Acid Binding Protein, Heart (FABP)
Description:
FABP is a plasma marker of acute myocardial infarction (AMI). The plasma kinetics of FABP (15kD) closely resemble those of myoglobin in that elevated plasma concentrations are found within 2 hours after AMI and return to normal generally within 18 to 24 hours. The concentration of FABP in the skeletal muscle is 20 times lower than in cardiac tissue (for myoglobin the same content for cardiac and skeletal tissue), making FABP cardiac-specific in comparison to myoglobin. This makes FABP a useful biochemical marker for the early assessment or exclusion of AMI. FABP also appears a useful plasma marker for the estimation of myocardial infarct size.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and for purification.

Recommended Dilution:
Western Blot: 1:50
Immunohistochemistry (Frozen and paraffin sections): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16A548Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Full length native human H-FABP (Purified)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA and 0.02% sodium azide.
Specificity:
Recognizes human heart FABP (native and recombinant). Displays no binding to human intestinal or liver FABP. Stains heart muscle cells and striated skeletal muscle cells in Immunohistochemistry. Can be used to detect ischemic areas of human heart. Crossreactivity: Moderate reactivity with human Brain Fatty Acid Binding Protein (B-FABP). Species Crossreactivity: rat and mouse H-FABP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Roos, W., et al., J. Immunol. Meth. 183: 149 (1995). 2. Guillame, E., et al., Proteomics 3: 1495 (2003). 3. Pelsers, M., et al., Clin. Chem. 50: 1568 (2004).