Technical Data
F0019-75L
Fatty Acid Binding Protein, Heart (FABP) (Biotin)
Description:
FABP is a plasma marker of acute myocardial infarction (AMI). The plasma kinetics of FABP (15kD) closely resemble those of myoglobin in that elevated plasma concentrations are found within 2 hours after AMI and return to normal generally within 18 to 24 hours. The concentration of FABP in the skeletal muscle is 20 times lower than in cardiac tissue (for myoglobin the same content for cardiac and skeletal tissue), making FABP cardiac-specific cf. myoglobin. This makes FABP a useful biochemical marker for the early assessment or exclusion of AMI. FABP also appears a useful plasma marker for the estimation of myocardial infarct size.

Applications:
Anti-human heart FABP is useful for immunoassays, Immunoprecipitation, Immunohistology of frozen sections, Western Blots and for purification.

Recommended Dilution:
Immunohistology: 1:10
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16A548Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified human H-FABP
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA. Conjugated to Biotin.
Specificity:
Recognizes human heart FABP. Shows no binding to human intestinal or liver FABP. Stains heart muscle cells and striated skeletal muscle cells in immunohistology. Can be used to detect ischemia areas of human heart. Crossreactive with rat and mouse H-FABP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Roos, W et al; Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Meth 1995, 183: 149.