Technical Data
F0019-82E
Fatty Acid Binding Protein 4 (FABP4, A-FABP)
Description:
Fatty acid binding proteins (FABPs) bind to fatty acids and other lipids to function as cytoplasmic lipid chaperones. They participate in the transport of fatty acids and other lipids to various cellular pathways. The predominant fatty acid binding protein found in adipocytes is FABP4, also called adipocyte fatty acid binding protein or aP2. FABP4 is also expressed in macrophages. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE). Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to the sequence of mouse Fatty Acid Binding Protein 4.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total mouse Fatty Acid Binding Protein 4 at ~15kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tuncman, G. et al. (2006) Proc. Natl. Acad. Sci. USA 103: 6970-6975. 2. Haunerland, N.H. and Spener, F. (2004) Prog. Lipid Res. 43: 328-349. 3. Makowski, L. et al. (2001) Nat. Med. 7: 699-705. 4. Hotamisligil, G.S. et al. (1996) Science 274: 1377-1379.