Technical Data
F0019-98G
FBG4 (F-box 4)
Description:
F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.

Applications:
Western Blot: Use at 1:500 to 1:1000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Residues with residues 70-85 (LQLARDRSAEGRALYA) of the human FBG4 protein. Crossreacts with mouse and rat FBG4 (100% sequence identity).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ilyin, G.P. et al. Gene. 2002 Aug 21;296(1-2):11-20.